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. 2018 Feb 9;10(2):72. doi: 10.3390/v10020072

Figure 1.

Figure 1

Zika virus (ZIKV) replication efficiency in favipiravir and ribavirin-treated human dermal fibroblasts (HDFs) and A549 cells. (A) HDFs, A549, and Vero cells were treated with various doses of favipiravir (μM), or ribavirin (μg/mL) and incubated for 24 h. At the end of incubation, cell viability was determined using MTT assays. Each value represents the mean ± SEM (n = 4). (B) HDFs and A549 cells were infected with ZIKV at MOI of 1 for indicated times. ZIKV E and NS5 expressions are shown. * p < 0.05; ** p < 0.01; *** p < 0.001, compared with mock control. (C) HDFs and (D) A549 cells were infected with ZIKV at MOI of 1 and treated with either a DMSO control (0 μM), favipiravir (1, 10, 25, and 50 μM), or ribavirin (1, 10, 25, and 50 μg/mL) for 24 h. qRT-PCR was performed to measure ZIKV E and NS5 mRNA levels. The expression of viral transcripts was calculated in relation to the expression level of GAPDH mRNA and expressed as fold-changes relative to expression levels in DMSO control-treated cells. * p < 0.05; ** p < 0.01; *** p < 0.001, compared with DMSO control-treated cells.