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. 2018 Feb 13;10(2):81. doi: 10.3390/v10020081

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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NHEJ-CRISPR/Cas9-based knock-in is non-directional and the unintended indels are minimal. (A) The schematic of sg2 target site in the intergenic region between DEV UL26 and UL27; (B) the target sequence of sgU; (C) the sequencing results of 5′ junction of 4 clones of DEV-GFP-HA; (D) the sequencing results of 3′ junction of 4 clones of DEV-GFP-HA. The DEV genome is colored in black; the sgU target sequence is colored purple; the insertion sequence is colored green; and the indel is colored in red.