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. 2018 Feb 13;10(2):81. doi: 10.3390/v10020081

Figure 4.

Figure 4

Figure 4

Optimization of GFP reporter removal via Cre-Lox system. CEF cells were transfected with Cre or control plasmids. At 24 h post transfection, the CEF cells were infected with DEV-GFP-HA at MOI 0.0025 or 0.01 respectively. Images were taken at 48 h post infection. (A) Empty vector with 0.0025 MOI infection; (B) Cre plasmid with 0.0025 MOI infection; (C) empty vector with MOI 0.01 infection; (D) Cre plasmid with 0.01 MOI infection; (E) the efficiency of GFP reporter removal with different infection DEV dose. The virus was harvested at 48 h post infection and was used to infect CEF cells; the percentage of GFP positive and negative plaques was calculated.