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. 2018 Feb 18;10(2):88. doi: 10.3390/v10020088

Figure 1.

Figure 1

Effect of knock down of piRNA-related transcripts in Aag2 cells on SFV. Aag2 cells were transfected with unique, gene-specific dsRNAs against Piwi4 (positive controls) and potential piRNA pathway proteins. (A) Knock down of Ae. aegypti homologs of Drosophila transcripts of the piRNA pathway using dsRNA (dseGFP as control). Gene-specific primers were used to confirm silencing and an actin PCR product was used as internal control; (B) Aag2 cells transfected with gene specific dsRNAs (dsRNA against eGFP was used as negative control) were infected with FFLuc-expressing SFV (MOI 0.01) and cells lysed 48 hpi. FFLuc activities were measured and normalized to these from control cells (taken as 1); the dotted line represents the threshold level of at least a two-fold increase in luciferase levels. The mean values and standard error of three experiments conducted independently in triplicate are shown; * denotes p < 0.05 (according to Student’s t-test).