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. 2018 Feb 18;10(2):88. doi: 10.3390/v10020088

Figure 3.

Figure 3

Effect of SpnE silencing on BUNV and ZIKV replication. dsRNAs were transfected into Aag2 cells to silence SpnE and cells treated with eGFP specific dsRNAs were used as control. At 24 hpt cells were infected with either Nanoluc-expressing BUNV-NL or wt ZIKV. (A) Cells infected with BUNV-NL (at MOI of 0.01) were lysed at 48 hpi; the relative mean luciferase activity values (normalized to these from dseGFP treated cells), together with the error of the mean from three independent experiments conducted in triplicate, are shown; (B) media was harvested at 72 hpi from wild-type ZIKV infected (MOI of 0.1) cells and subjected titration. The mean virus titre together with standard error of 6 independent experiments are shown. “n. s” indicates non-significance according to Student’s t-test.