Skip to main content
. 2018 Feb 18;10(2):88. doi: 10.3390/v10020088

Figure 4.

Figure 4

Effect of SpnE knock down on the siRNA pathway based silencing efficiency. Aag2 cells were first treated with dsRNA against eGFP (control), Ago2, Piwi4 or SpnE. 24 h later the cells were co-transfected with FFLuc and Rluc expressing reporter plasmids (pIZ-Fluc and pAcIE1-Rluc) and siRNAs against FFLuc (siFFLuc) or Hygromycin B (siHyg) (A) to assess the silencing ability of pre-formed siRNAs in the cells, or alternatively dsRNAs targeting FFLuc (dsFFLuc) or mCherry (dsmCherry) (B) to assess the silencing ability of siRNAs produced in a Dcr2-dependent manner. Cells were lysed at 24 hpt and activities of luciferases determined. Relative luciferase levels are shown on the Y-axis (with FFLuc/Rluc ratio in siHyg or dsmCherry transfected cells set to 1). The mean values with standard error are shown for three independent experiment conducted in triplicate, * shows p < 0.05 using two-way ANOVA.