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. 2018 Feb 18;10(2):88. doi: 10.3390/v10020088

Figure 5.

Figure 5

Northern blot analysis of virus-specific piRNAs. Aag2 cells were transfected with dsRNAs against Ago3, Piwi5, Piwi6, SpnE or eGFP (as negative control). 24 h pt the cells were infected with wt SFV (A) or wt CHIKV (B) at a MOI of 10. Total RNA isolated at 48 hpi was separated on PAGE-Urea gel, transferred onto nylon membrane and probed with virus specific radiolabelled DNA oligos for piRNAs and siRNAs. In the left panel, a representative image of three independent experiments is shown; in the right panel the mean levels of three independent experiments with standard deviation of SFV- or CHIKV-specific vpiRNAs are shown, * indicates significance, p < 0.05 (Student’s t-test); vpiRNA levels in control dseGFP treated cells taken as 100%.