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. 2018 Feb 23;10(2):90. doi: 10.3390/v10020090

Figure 2.

Figure 2

Reverse genetics use in making customized VSV targeting tumor lysis. Schematic presentation of VSV reverses genetics system. Plasmid DNA harboring full-length viral genome and other accessory proteins are transfected to the supporting cell line to initiate virus genome transcription and replication. A helper virus or another plasmid expressing T7 polymerase is required to initiate transcription. Later, the accessory proteins (N, P, and L) takeover viral genome replication and transcription leading to the recovery of new viruses. pVSVFL: plasmid containing cDNA copy of VSV full-length genome.