Figure 1.

Miniaturized luciferase reporter gene assay in 96-well plates. (a) Human embryonic kidney 293T (HEK293T) cells were transfected with 15, 30, 60, and 240 ng of pISRE-luc per well. Twenty-four hours after transfection, cells were stimulated (S) or not (NS) with interferon-α (IFN-α) for IFN signaling pathway activation. After 8 h, cells were lysed and luciferase activity was measured; (b) HEK293T cells were transfected with 60 ng/well of pISRE-luc. Twenty-four hours after transfection, cells were stimulated with IFN-α. After 4, 8, 12, 24, 48 h, cells were harvested and luciferase activity was measured. Results are shown as pISRE-luc fold induction of stimulated cells over the not-stimulated control. Error bars indicate the mean ± SD.