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. 2018 Feb 24;10(2):98. doi: 10.3390/v10020098

Figure 5.

Figure 5

Z′- and Z-factor determination for IFN-α stimulation and VP24 inhibition assays. Firefly luciferase activity is normalized to the Renilla luciferase internal control. Results are expressed as pISRE-luc fold induction over the unstimulated control. The Z′- and Z-factor were calculated as described in methods. (a) HEK293T cells were seeded in a 96-well plate and transfected with pISRE-luc (60 ng/well) and pcDNA3.1 (30 ng/well). Twenty-four hours post-transfection, 48 wells (S) were incubated with 1 ng/mL of IFN-α and 48 wells were left untreated (NS). (b) HEK293T cells seeded in 48 wells were transfected with pISRE-luc (60 ng/well) and pcDNA3.1 (30 ng/well), and those in the remaining 48 wells were transfected with pISRE-luc (60 ng/well) and expression plasmid for VP24 (30 ng/well). Thirty-five wells for each sample were stimulated with 1 ng/mL of IFN-α and 12 were not stimulated.