Fig. 2.

Paired read evidence for a reciprocal translocation involving chromosomes 01 and 04. (A) Circos representation of significant discordant read clusters from PT-BA-00267 identified in the targeted regions of reference chromosomes 01 (0–8.5 Mb) and 04 (23.5–29.5 Mb). (B) Circos with focus on paired reads in a 25 kb region around the discordant read cluster detected in chromosomes 01 and 04 in (A). Grey lines correspond to concordant pairs (correct orientation and insert size), orange and red lines correspond to discordant pairs with smaller and greater insert sizes, respectively. Purple lines correspond to pairs showing a reverse–reverse orientation, green lines a forward–forward orientation, and blue lines correspond to pairs with a complete reverse orientation relative to the paired library construction. (C) Circos with focus on paired read clusters detected in the targeted region of chromosomes 01 and 04 and with chromosome 08. (D) Hypothesized chromosome structures for PT-BA-00267 based on the paired read mapping interpretation. Linked colored arrows correspond to the read pairs shown in (C). Centromeres are indicated by circles.