Table 1.

Identification of PONDR-VLXT Predicted VPg-SON41p Disorder-Affecting Amino Acid Substitutions Based on the VPg PVY (group C) Natural Genetic Diversity.

			PVY SON41p
			93	94	95	96	98	100	101	102	105	108	114	115	117	118	119	121	123
			I	R	D	I	E	F	S	E	R	V	E	T	A	L	D	H	S
VPg group C polymorphic sites	93	V	–
	94	G		d
	95	E, N			d
	96	V				–
	98	D					–
	100	L						d
	101	G							RB
	102	Q								o
	105	K									RB*
	108	I										–
	114	Q											o
	115	A, M, R, T												RB
	117	V													–
	118	V														–
	119	S, E, H															RB
	121	N																RB*
	123	K, E, N, T																	RB*

Second and third line/column: polymorphic amino acid sites and their corresponding positions in VPg protein sequence. Red/blue boxes: amino acids substitutions that are predicted (PONDR-VLXT) to enhance or reduce intrinsic disorder content in VPg-SON41p respectively. “d” and “o”: respectively intrinsic disorder and order promoting substitutions; “RB”: pvr2³-resistance breaking sites previously identified (Ayme et al. 2006; Montarry et al. 2011); “RB*”: expected resistance-breaking sites (displaying high dN/dS ratios; Ayme et al. 2007; Moury et al. 2014).