Fig. 3.

Sexually dimorphic 20-hydroxyecdysone titers, but not doublesex isoforms, are associated with cell division and larger EcR expression domains in late Wr stage eyespot centers. (A) (i) dsx mRNA is present in the pheromone producing organ of males (yellow box) but is absent from the eyespot centers (arrows). N = 4 for in situ stainings. (ii) Male forewing with male pheromone producing organ (iii). (B) 20E titers observed during fine intervals of wandering (Wr) and prepupal (PP) stages. Error bars represent 95% CI of means. (C) Larval wings immunostained with EcR (Red) and pH3 (Green) antibodies at two stages of Wr stage—40% and 90% development, zoomed in to show the developing dorsal Cu1 eyespot centers (fig. 1C). Scale bars, 20 μm.