Fig. 4.
The advantage of replication over high sequencing coverage. Allele frequency tajectories were simulated for 100,000 unlinked loci over 60 generations assuming Ne = 300 with p0 ∈ [0, 1] and s ∈ [0, 0.3]. Selection coefficients were estimated with LLS using either a single replicate per locus (green), or by combining the information of multiple replicates (purple). The overall sequencing effort was assumed to be constant. The relative deviation between estimated and true selection coefficient is shown as a function of s (A) and p0(B).