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. 2018 Mar 5;131(5):544–552. doi: 10.4103/0366-6999.226063

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Copyright: © 2018 Chinese Medical Journal

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The expression of VEGF in heart at the levels of protein and mRNA. (a) Representative gel of four independent experiments. (b) The gray scale analysis of scanned gel images. Data have shown that the VEGF protein expression has no significant changes between ApoE-/- mice and C57BL/6J mice (0.83 ± 0.11 vs. 0.78 ± 0.06; F = 5.298, P > 0.05), but it was enhanced in ApoE-/- + TXL mice (1.42 ± 0.43 vs. 0.83 ± 0.11; F = 20.247, *P = 0.004). (c) TXL enhanced VEGF mRNA expression. VEGF mRNA levels were quantified by real-time PCR and normalized to GAPDH mRNA. As the expression of protein, VEGF mRNA expression has no change between ApoE-/- mice and C57BL/6J mice, but it was enhanced by TXL up to 4.59-fold in ApoE-/- mice (F = 17.489, *P = 0.003). VEGF: Vascular endothelial growth factor; TXL: Tongxinluo.