Table 1.

Primers and probes, with final concentrations, used in TaqMan Multiplex QPCR assay for quantification of rickettsial levels

Primer/probe name	Sequence (5′ → 3′)	Final concentration
QrompB_F	AAGTGGTACTTCAACATGGG	400 nM
QrompB_R	GCACCACCTTGGATTAAAG	400 nM
CaRa_probe_FAM	ATCGCGGAAGGTGCTCAAGTTAATG	50 nM
Rp_probe_HEX	ATTTTGGAAGGTGCGCAAGTTAATGC	400 nM
Amac MIF.18F	CCAGGGCCTTCTCGATGT	300 nM
Amac MIF.99R	CCATGCATTGCAAACC	300 nM
Amac MIF.63_Cy5	TGTTCTCCTTTGGACTCAGGCAGC	200 nM

Rickettsial ompB sequences used for design of primers and probes were GenBank accession numbers GU131157 and AF123717.