Table 2.

Number of A. macluatum [male:female] positive for rickettsial DNA [male:female] out of total A. maculatum collected per site in Oktibbeha County, MS, over the 3-yr collection period

Site no.	2013			2014			2015
	R. parkeri	“Ca. R. andeanae”	Coinfected	R. parkeri	“Ca. R. andeanae”	Coinfected	R. parkeri	“Ca. R. andeanae”	Coinfected
1	16 [6:10]/45 [18:27]	0 [0:0]/45 [18:27]	0 [0:0]/45 [18:27]	15 [9:6]/66 [35:31]	12 [4:8]/66 [35:31]	2 [1:1]/66 [35:31]	13 [7:6]/42[22:20]	5 [4:1]/42[22:20]	0 [0:0]/42[22:20]
2	17 [8:9]/50 [23:27]	0 [0:0]/50 [23:27]	0 [0:0]/50 [23:27]	2 [0:2]/10 [1:9]	0 [0:0]/10 [1:9]	0 [0:0]/10 [1:9]	0 [0:0]/1[0:1]	0 [0:0]/1[0:1]	0 [0:0]/1[0:1]
3	NSa	NS	NS	NS	NS	NS	31[13:18]/58[24:34]	11[4:7]/58[24:34]	1[0:1]/58[24:34]
4	NS	NS	NS	2 [1:1]/63 [37:26]	3 [2:1]/63 [37:26]	0 [0:0]/63 [37:26]	NS	NS	NS
Total	33 [14:19]/95 [41:54]	0 [0:0]/95 [41:54]	0 [0:0]/95 [41:54]	19 [10:9]/139 [73:66]	15 [6:9]/139 [73:66]	2 [1:1]/139 [73:66]	44 [20:24]/101[46:55]	16 [8:8]/101[46:55]	1 [0:1]/101[46:55]

Tick extracts initially positive by rompB QPCR were confirmed by rompA amplicon sequencing (2015 samples) and final QPCR analysis for rickettsial levels (2013–2015).

^aNot sampled.