Figure 2.

eGFP is expressed in transplanted testis, but not in benign and malignant lesions outside of the testis. Gel electrophoresis of a standard PCR for eGFP performed on genomic DNA from adult testis and lesions found in transplanted animals. Genomic DNA of adult B6D2F1 actin-eGFP testis serves as a positive control. Mouse 541570 serves as an example for all transplanted mice, and was transplanted unilaterally with eGFP positive GS cells, which can be confirmed by PCR 14 months after transplantation with a positive eGFP signal in the transplanted testis and no signal in the contralateral untransplanted testis. Mouse 541555 developed a hematoma in the transplanted testis, explaining a positive read-out in this case. eGFP could not be detected in all other benign and malignant lesions. An amplicon for β-actin is present in all samples. LN, lymph node; LCL, diffuse large cell lymphoma.