Table 1.
Antibody characteristics used in single- and double-label immunocytochemistry.
| Target antigen | Catalog Number | Manufacturer; species & type | Antibody class | Immunogen | Dilution | Citation/Reference |
|---|---|---|---|---|---|---|
| Fra-2 | Sc-604 | Santa Cruz; rabbit polyclonal | IgG | N-terminus of Fra-2 | 1:1000 | (Nishina et al., 1990; Suzuki et al., 1991; Peczely and Kovacs, 2000) |
| Tyrosine hydroxylase | 22941 | Immunostar, Mouse monoclonal | IgG1 | TH from Rat PC-12 cells | 1:2000 | (Goodson et al., 2012; Kang et al., 2010; Riters et al., 2000; Riters et al., 2007) |
| Opsin clone Ret-P1 | O 4886 | Sigma-Aldrich; mouse monoclonal | IgG1 | Amino acid residues 4–10 at N-terminus | (Silver et al., 1988; Saldanha et al., 2001) | |
| Opsin-5 | N/A | Gift from Takashi (Nakane et al., 2010), PhD; rabbit polyclonal | IgG | C-terminal region of OPN-5 | 1:1000 | (Nakane et al., 2010) |
| melanopsin | N/A | Gift from Muhamad El Halawani; rabbit polyclonal | IgG | 1:500 | (Kang et al., 2010) |