Fig. 1.

Western blot analysis of anti-carbamylated protein antibodies

Carbamylated and unmodified fetal calf serum (FCS) samples were electrophoresed and blotted onto a nitrocellulose membrane, and then incubated with sera from seven RA patients containing a high titre of anti-carbamylated protein (anti-CarP) antibodies or anti-BSA polyclonal antibody. (A) The common band detected in sera from RA patients corresponded to carbamylated albumin. (B) Western blotting was also performed using unmodified and carbamylated BSA.