Table 2.
Calculated and analyzed nutrient and energy content of the experimental diets.
| Description of diets | PC1 | PC500 | PC1,500 | NC2 | NC500 | NC1,500 |
|---|---|---|---|---|---|---|
| Phytase, FTU/kg | 0 | 500 | 1,500 | 0 | 500 | 1,500 |
| Calculated nutrients and energy: | ||||||
| Protein, g/kg | 230.5 | 230.5 | 230.5 | 230.3 | 230.3 | 230.3 |
| ME, kcal/kg | 3,020 | 3,020 | 3,020 | 2,941 | 2,941 | 2,941 |
| Ca, g/kg | 10.0 | 10.0 | 10.0 | 8.4 | 8.4 | 8.4 |
| P, g/kg | 6.9 | 6.9 | 6.9 | 5.5 | 5.5 | 5.5 |
| Available P, g/kg | 5.0 | 5.0 | 5.0 | 3.5 | 3.5 | 3.5 |
| Ca:P | 1.4 | 1.4 | 1.4 | 1.5 | 1.5 | 1.5 |
| Na | 1.8 | 1.8 | 1.8 | 1.7 | 1.7 | 1.7 |
| K | 7.9 | 7.9 | 7.9 | 7.9 | 7.9 | 7.9 |
| Cl | 2.2 | 2.2 | 2.2 | 2.2 | 2.2 | 2.2 |
| Mg | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 |
| Total amino acids, g/kg | ||||||
| Lys | 14.0 | 14.0 | 14.0 | 14.1 | 14.1 | 14.1 |
| Met | 5.0 | 5.0 | 5.0 | 5.0 | 5.0 | 5.0 |
| Cys | 4.0 | 4.0 | 4.0 | 4.0 | 4.0 | 4.0 |
| Phe + Tyr | 17.5 | 17.5 | 17.5 | 17.5 | 17.5 | 17.5 |
| Analyzed nutrient content (g/kg): | ||||||
| Ca | 12.2 | 11.1 | 10.7 | 10.8 | 10.4 | 10.2 |
| P | 7.5 | 6.7 | 7.1 | 6.1 | 5.8 | 5.7 |
| Ca:P | 1.6 | 1.6 | 1.5 | 1.8 | 1.8 | 1.8 |
| Phytate-P | 1.7 | 2.4 | 2.2 | 1.7 | 2.0 | 1.8 |
| Crude protein3 | 3.54 | 3.55 | 3.45 | 3.44 | 3.58 | 3.66 |
| IP6 (mg/kg) | 11.54 | 11.53 | 11.56 | 11.07 | 11.14 | 11.93 |
| IP5 (mg/kg) | 0.558 | 0.574 | 0.595 | 0.629 | 0.665 | 0.661 |
| Na | 1.8 | 1.7 | 1.5 | 1.7 | 1.6 | 1.6 |
| K | 9.0 | 8.5 | 9.7 | 9.0 | 8.9 | 8.5 |
| Mg | 1.4 | 1.3 | 1.5 | 1.4 | 1.4 | 1.3 |
| Phytase4,5,6 (FTU/kg) | <50 | 715 | 1,880 | <50 | 744 | 1,960 |
1PC: Positive control.
2NC: Negative control.
3Crude protein: Nitrogen analyses × 6.25.
4The enzyme used was Quantum Blue, provided by AB Vista, with an initial activity level of 5,000 FTU/kg. The premix was made to 150 FTU/kg and added at the rate of 10 g/kg or 30 g/kg to give activity levels of 500 or 1,500 FTU/kg, respectively.
5Dietary phytase quantification determined using ELISA method by AB Vista on final diets.
6Enzyme premix quantified at 56 FTU/kg using Quantum method by AB Vista.