Figure 3.

Relative amounts of adiponectin target genes in blastocysts stimulated in vitro with adiponectin for 4 h. Transcription analyses of adaptor protein (APPL1), peroxisome proliferator-activated receptor alpha (PPARα), CD36, fatty acid transporter 4 (FATP4), fatty acid binding protein 4 (FABP4), hormone sensitive lipase (HSL) and lipoprotein lipase (LPL) were performed by RT-qPCR in blastocysts cultured in vitro with adiponectin (1 μg/ml) for 4 h. Culture was performed in groups of at least 4 and repeated in three independent replicates (N = 3 (number of individual and independent experiments); n ≥ 4 (number of samples used per treatment group in each independent experiment, per sample four blastocysts have been pooled)). Values are expressed as fold change relative to the treatment control (set 100% and indicated with the dashed line) (*P < 0.05, Student's t-test).