Table 5.

Significant upstream transcriptional regulators in the diabetic rat model

Upstream Regulator	Molecule Type	Predicted Activation State	Activation z-score	Target molecules in dataset
RABL6	Other	Inhibited	−2,010	ABAT,AURKB,BIK,CCNB1,DAPK1,H2AFX,MCM5,MCM7,RFC3,SERPINH1,TOP2A,TP53
MAX	Transcription regulator	Inhibited	−2,000	CCND2,CDKN2B,FBXO32,GADD45B,KLF10,TMEM126A,TXNIP
HLX	Transcription regulator	Inhibited	−2,000	BMP2,CCNB1,CYP1B1,GDF15
IFNL1	Cytokine	Inhibited	−2,186	EIF2AK2,IFI35,MX1,UBE2L6,USP18
MNT	Transcription regulator	Inhibited	−2,000	FBXO32,GADD45B,KLF10,TXNIP
IFNG	Cytokine	Inhibited	−2,668	ALOX15,ASS1,BCL2L1,CASP8,CAT,Ccl2,CCL22,CYP2E1,DDR2,DPP4,EIF2AK2,ICOSLG/LOC102723996,IFIT1B,LOX,MX1,NOS2,NPY2R,SDC4,SERPINB9,SLC15A3,STAR,TGM1,TXNIP,UBD,USP18
IFNA2	Cytokine	Inhibited	−2,068	EIF2AK2,IFI35,IFNB1,MX1,TP53,UBE2L6,USP18
PCDH11Y	Other	Inhibited	−2,236	CCND1,CCND3,CD44,GJA1,RET
SPDEF	Transcription regulator	Activated	2,496	CDH11,COL1A1,COL4A1,COL4A2,COL5A1,COL5A2,COL6A1,COL6A3,DKK3,EGFR,ITGA6,LAMC1,PRKCA,SMAD3
KIAA1524	Other	Activated	2,359	C4BPA,CDKN2B,COL8A1,ENO3,GPNMB,LUM,RHOC,SERPINE2,TUBA4A
miR-29b-3p (and other miRNAs w/seed AGCACCA)	Mature microrna	Activated	2,449	COL1A2,COL5A2,GAS7,LAMC1,MYBL2,TUBB2A
CST5	Other	Activated	2,584	ACAT2,AKAP12,AP1M2,CAV1,CD44,DECR1,DRAP1,ELK3,EMP3,EPN3,FER,FHL1,LAMC1,NR2F1,NRP1,PLS3,PRSS8,S100A11,TAF1,TGIF1,TSPAN5,VCAN
PTGS2	Enzyme	Activated	2,425	BCL2L1,Ccl2,IFNB1,ITGA6,MCL1,MMP14,PPA1,TP53
SIRT1	Transcription regulator	Activated	2,049	ATXN10,BNIP3,CCND2,CPT1A,CYP1A1,HMGCR,IGF1,TP53
KITLG	Growth factor	Activated	2,000	BCL2L1,IL1RL1,PRKCA,PRKCB
FOXO3	Transcription regulator	Activated	2,197	BNIP3,CAT,CCND2,CDKN2B,EGR4,FBXO32,GADD45B,MXI1,TXNIP
NEDD9	Other	Activated	2,646	BMP2,BNIP3,ELF3,FABP1,GDF15,MMP14,TXNIP
POU2F2	Transcription regulator	Activated	2,236	ALDOC,KCNN4,MCM7,PFKFB3,SSTR2,VIP

Orange/blue indicate the predicted activation state, namely activated/inhibited, of the transcriptional regulator.