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. Author manuscript; available in PMC: 2018 Mar 14.
Published in final edited form as: J Phys Chem B. 2016 Dec 5;120(49):12420–12431. doi: 10.1021/acs.jpcb.6b08323

Figure 3.

Figure 3

Differences in chemical shifts between MgCl2(0NaCl) and MgCl2(50NaCl), labeled as Δδ(0 – 50 NaCl), at 5 mM (A), 20 mM (B), and 80 mM (C) MgCl2, and (D) chemical shift perturbation, Δδ(ppm), at 160 mM NaCl concentration detected using the 0 MgCl concentration points of MgCl2(0NaCl) and Mg2+(const. Cl−). A horizontal arrow in the top panel indicates the location of the substrate handle-region (residues 503–527). Asterisks (*) indicate unassigned or overlapped backbone NH amides, and P indicates prolines.