Plots of Δδ as a function of Mg2+ concentration for residues (A) K512, (B) E514, and (C) S515 at the substrate-handle region of RNH. Experimental data points for MgCl2(0NaCl), MgCl2(50NaCl), and Mg2+(const. Cl−) are represented by open square, closed triangle and cross symbols, respectively. Fit curves calculated assuming 1:1 interaction are shown by dotted, dashed, and solid lines for MgCl2(0NaCl), MgCl2(50NaCl), and Mg2+(const. Cl−), respectively.