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. 2018 Feb 28;2018:1047810. doi: 10.1155/2018/1047810

Figure 2.

Figure 2

DMY attenuates TNF-α-impaired tube formation and migration in a miR-21 and NO-dependent manner in HUVECs. (a) Representative images show the effect of DMY, miR-21 agomir and antagomir, and L-NAME on tube formation induced by TNF-α (magnification 20x; scale bar, 150 μm); (b) representative images show the effect of DMY, miR-21 agomir and antagomir, and L-NAME on migrated cells on transwell membranes induced by TNF-α (magnification 20x; scale bar, 150 μm). (c) The number of tubes was counted in (a). (d) The number of migrated cells was counted in (b). (e) The transfected efficiency of miR-21 agomir and antagomir on miR-21 expression in HUVECs treated with TNF-α. Data was expressed as mean ± SD, n = 3, ∗∗P < 0.01 versus control, ##P < 0.01 versus TNF-α, &&P < 0.01 versus TNF-α + DMY (25 μM), and ++P < 0.01 versus TNF-α + miR-21 antagomir. ∗∗P < 0.01 versus agomir NC, $$P < 0.01 versus antagomir NC, ##P < 0.01 versus TNF-α + miR-21 agomir NC, and ΦΦP < 0.01 versus TNF-α + miR-21 antagomir NC in (e).