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Knockdown of Smurf2 decreased the level of endogenous CNKSR2. a Representative images of MDA-MB-231 cells expressing either control or Smurf2 shRNA constructs. b qRT-PCR analysis of Smurf2 shRNA knockdown cells indicated that Smurf2 depletion had little to no effect on CNKSR2 mRNA levels. (c, d) Depletion of Smurf2 leads to decreased CNKSR2 expression in MDA-MB-231cells. Results represented as mean ± standard deviation from three independent experiments
