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. 2018 Mar 13;19:2. doi: 10.1186/s12867-018-0103-x

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© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Different resolution of defined glomerular structures in dependence on deparaffinization. Without deparaffinization (A, B), only coarse structures are visible. However, deparaffinization and a short hemalaun stain allow the detection of nuclei of PECs (green arrowheads) and endothelial (red arrowheads) cells (C, D). By contrast, globally (E, black arrow) or segmentally (F, black arrow) sclerosed glomeruli as well as scarred tubulo interstitium (E, black arrowhead) can easily be detected without deparaffinization. One glomerulus with open capillary loops is shown in comparison (F, green arrow). HE (G) and PAS (H, I) stains from consecutive sections of D–F are given. PALM/Zeiss laser technique and software, magnification ×400 or ×200, C, D short hemalaun stain