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. Author manuscript; available in PMC: 2018 Nov 1.
Published in final edited form as: Clin Cancer Res. 2017 Jul 27;23(21):6650–6660. doi: 10.1158/1078-0432.CCR-17-0120

Figure 3. Correlation of GBM IDO1 mRNA levels with IDH mutation, DNA methylation and immunosuppressive gene expression.

Figure 3

(A) IDO1 mRNA levels were compared among classical (blue circle), mesenchymal (red square) and neural (green triangle) GBM subtypes analyzed from the cancer genome atlas RNA-Hi-Seq. Illumina database. The Proneural GBM subtype was further stratified into IDH1/2-wild-type (IDHwt; purple circle) and IDH1/2-mutant (IDHmut; orange square) samples. (B) IDO1 mRNA levels were compared among grade II (IDHwt; blue circle and IDHmut; red square), grade III (IDHwt; green triangle and IDHmut; purple circle), as well as grade IV (IDHwt; orange circle and IDHmut; black triangle) glioma. (C) DNA methylation analysis of cg10262052, cg08465774 and cg24188163 in grade II (IDHwt; blue circle and IDHmut; red square), grade III (IDHwt; green triangle and IDHmut; purple circle), as well as grade IV (IDHwt; orange circle and IDHmut; black triangle) glioma. Pearson’s correlation analysis for IDO1 mRNA with PD-L1, PD-L2, PD-1, CTLA-4, STAT3, CD39, BTLA, LAG3, FOXP3 and FGL2 in (D) GBM and (E) pooled grade II and III glioma. Each small circle in the plot represents a single patient data point. For all the scatter plots, horizontal lines represent mean ± SEM. ***, P < 0.001, ****, P < 0.0001.