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. Author manuscript; available in PMC: 2018 May 30.
Published in final edited form as: Nature. 2017 Nov 22;551(7682):648–652. doi: 10.1038/nature24661

Figure 3. Metabolite profiling reveals shared and divergent features in AAA metabolic pathways.

Figure 3

Mutant and wild-type C. sporogenes were incubated with phenylalanine, tyrosine and tryptophan derivatives of each of the four coloured compounds and metabolites were assayed by LC-MS/MS. Compared to wild-type, the fldH, fldC and acdA mutants were deficient in reductive metabolism of phenylalanine, tyrosine and tryptophan (rows 1–4, column 5, red and green traces). Relative to wild-type, the porA mutant was deficient in oxidative metabolism of phenylalanine and tyrosine, but not tryptophan (rows 1 and 5, column 1, red and green traces). Representative chromatograms from n = 3 biological replicates. Phe, phenyl; 4-OH-Phe, 4-hydroxyphenyl.