Extended Data Figure 2. Generation of the phenyllactate dehydratase disruption mutant.

a, The ClosTron bacterial type II intron system was used to disrupt fldC (CLOSPO_00311) by insertion of an erythromycin-containing intron within the coding sequence at nucleotide position 561. b, Successful integration at the fldC locus was determined by amplifying DNA using primers flanking the region of insertion. The expected PCR product for the wild type is 600 bp as shown in the ethidium-bromide-stained agarose gel from a single experiment. Successful chromosomal integration was confirmed by Sanger sequencing.