Extended Data Figure 4. acdA, fldH and porA mutants exhibit growth defects when cultured with amino acids as the sole carbon source.

a–f, The wild-type (a–f) and acdA (a, d), fldH (b, e) and porA (c, f) mutant strains of C. sporogenes were cultured in minimal defined medium containing 10 amino acids at standard concentrations (SACC) with (d–f) or without (a–c) glucose (10 mM) and growth was monitored spectrophotometrically. Representative curves from n = 3 biological replicates are shown and doubling times are reported as mean ± s.d.