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. Author manuscript; available in PMC: 2018 May 30.
Published in final edited form as: Nature. 2017 Nov 22;551(7682):648–652. doi: 10.1038/nature24661

Extended Data Figure 6. The fldZ mutant is defective in conversion of phenylacrylate and 4-OH-phenylacryalte.

Extended Data Figure 6

Resting cell suspensions of wild-type and fldZ mutant C. sporogenes were prepared and incubated with phenylacrylate, 4-OH-phenylacrylate or indoleacrylate for 1 h at 37 °C. Cells were then collected by centrifugation and the corresponding arylacrylates were detected in the supernatant by LC-MS/MS. The presence of a peak corresponds to a defect in the conversion of the indicated substrate (that is, retention of precursor). Representative chromatograms from n = 3 biological replicates are shown.