Figure 3. Luciferase reporter assay suggests miR-491-3p directly targets 3′-UTR of ABCB1.
A) Overexpression with miR-491-3p mimic led to a 40% decrease in ABCB1 3′-UTR-luciferase activity in Caco-2 cells. Inhibition of miR-491-3p by selective inhibitor resulted in approximately 50% increase in luciferase activity in Caco-2 cells. B) Inhibition of miR-491-3p reversed atorvastatin attenuation of ABCB1 3′-UTR – luciferase activity. *p< 0.05, compared with corresponding control, #p<0.05 as compared with atorvastatin without inhibitor (n=6 in each group).