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. Author manuscript; available in PMC: 2018 Mar 14.
Published in final edited form as: Eur J Pharm Sci. 2016 Aug 26;93:431–436. doi: 10.1016/j.ejps.2016.08.044

Figure 4. ABCB1 expression is down regulated after 48h-atorvastatin treated cells and inhibition of miR-491-3p restores atorvastatin – induced decreases in ABCB1 levels in Caco-2 cells.

Figure 4

(A) Caco-2 cells were treated with atorvastatin for 48h and ABCB1 mRNA and protein expression were measured by RT-qPCR and western blot, respectively. (B) Caco-2 cells were transfected with miRNA-491-3p miRIDIAN hairpin inhibitor (25 nM) (miR-491-3p inhibitor) or miRIDIAN hairpin inhibitor negative control (25nM) (negative control) and treated or not with atorvastatin (atorva). ABCB1 expression was measured by western blot. Results are expressed as relative expression to negative control (vehicle-treated cells and transfected with miRIDIAN inhibitor negative control), *p< 0.05, compared with negative control.