Table 2.

Primer sequences used for the amplification and SNaPshot multiplex reaction of Calliphoridae and Sarcophagidae COI genes.

Family	Name	Sequence	Binding site
Calliphoridae	CA-SNP-F	5′-CAGTCTATTGCCTAAACTTCAG-3′	tRNA-tyrosine
	CA-SNP-R	5′-GTTARTGCRGGRGGTAAAAGTCA-3′∗	301–323 on COI
Sarcophagidae	SA-SNP-F	5′-AAGTTTAGYATCHCAACGWCAAGT-3′∗	1416–1439 on COI
	SA-SNP-R	5′-TTAAACCCATTGCACTAATCTGCC-3′∗	1543–1566 on COI

^∗Degenerated primers were used to detect target SNPs based on IUPAC nucleic acid sequences.