TABLE. Termocycling parameters for the real-time reverse transcriptase polymerase chain reaction (qRT-PCR).

Stage	Temperature	Duration	N° of cycles
Reverse transcription	50°C	15 min	1
Enzyme activation	95°C	2 min	1
Denaturation	95°C	15 s	40
Hybridisation/extension	60°C	1 min	40