TABLE. Termocycling parameters for the real-time reverse transcriptase polymerase chain reaction (qRT-PCR).
Stage | Temperature | Duration | N° of cycles |
---|---|---|---|
Reverse transcription | 50°C | 15 min | 1 |
Enzyme activation | 95°C | 2 min | 1 |
Denaturation | 95°C | 15 s | 40 |
Hybridisation/extension | 60°C | 1 min | 40 |