Table 4.

Summary of DRB variation in the three R. roxellana populations

Locus	Pop	N	k	Nu	Co	ABS	Variable nucleotide positons	Variable codons	Variable ABS	PIS	Probability test	P null	A R	PIC	A E	H E	H O	Overall mean distance	F IS	Tajima’s D (P-value)
DRB1	FP	32	6	243	81	18	48	29	16	27	0.034 ± 0.000	−0.010	5.994	0.542	2.338	0.581	0.563	0.101 ± 0.015	0.033	0.643(P > 0.10)
	NS	36	7	243	81	18	54	29	16	26	0.170 ± 0.000	0.072	6.993	0.606	2.692	0.637	0.583	0.105 ± 0.015	0.086	0.519(P > 0.10)
	ZZ	54	8	243	81	18	54	30	16	41	0.038 ± 0.000	0.026	6.899	0.701	3.878	0.749	0.703	0.111 ± 0.014	0.061	1.062(P > 0.10)
	All	122	11	243	81	18	60	30	16	43	–	–	8.030	0.665	2.969	0.697	0.631	0.104 ± 0.015	–	0.977(P > 0.10)
DRB2	FP	30	4	243	81	18	37	19	8	8	0.109 ± 0.000	−0.056	4.000	0.479	2.064	0.524	0.533	0.092 ± 0.019	0.000	0.506(P > 0.10)
	NS	36	6	243	81	18	40	22	11	22	0.368 ± 0.001	0.065	5.993	0.589	2.618	0.627	0.583	0.082 ± 0.014	0.070	0.564(P > 0.10)
	ZZ	53	6	243	81	18	42	21	11	18	0.000 ± 0.000a	0.202	5.530	0.579	2.730	0.640	0.415	0.084 ± 0.016	0.353	0.326(P > 0.10)
	All	122	8	243	81	18	44	23	12	28	–	–	6.352	0.584	2.471	0.621	0.496	0.082 ± 0.014	–	0.608(P > 0.10)

Pop population, N number of genotyped samples, k number of alleles, Nu nucleotides, Co codons, PIS parsimony-informative sites; Probability test were apply for testing deviation from the Hardy-Weinberg equilibrium (HWE); P_null, the frequency of null alleles; A_R, allelic richness; PIC, polymorphic information content; A_E, effective number of alleles; H_O and H_E, observed and expected heterozygosity, respectively; the overall mean distance here was applied to the nucleotide sequence; F_IS, inbreeding coefficient

^a, Excess of homozygotes at DRB2 locus for ZZ population