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CRISPR–Cpf1 mediated genome editing in the Pseudomonas putida KT2440. a Overview of the genome editing by CRISPR–Cpf1 in P. putida KT2440. b The schematic showing the design of identification primers to confirm genome editing in KT2440. c Agarose gel electrophoresis and the result of DNA sequencing show that pCpf1-RK2K enables gene deletion in KT2440
