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. 2001 Sep 4;98(19):10596–10601. doi: 10.1073/pnas.191159298

Figure 4.

Figure 4

Characterization of a binary patterned CM. (A) Size-exclusion chromatography traces of H1/H2/H3–12 (solid line) and MjCM′ (dashed line), showing similar retention times. MjCM′ was previously shown to be homodimeric by analytical ultracentrifugation (13). (B) The CD spectrum of H1/H2/H3–12. The molar ellipticities at 208 and 222 nm are comparable to those of WT MjCM′ (data not shown) and show that the protein is highly helical. (Inset) The cooperative thermal denaturation of H1/H2/H3–12.