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. 2018 Mar 11;17:1533033818762680. doi: 10.1177/1533033818762680

Figure 2.

Figure 2.

The antitumor roles of inhibitor of growth family of protein 5 (ING5) in osteosarcoma (OS) cells. (A) ING5 messenger RNA (mRNA) and protein levels in OS cells using reverse transcription PCR (RT-PCR) and Western blot, respectively. (B) The proliferation ratio of the cells was assayed using the soft agar assay. (C) Apoptotic cells were assayed by Annexin-V/FITC and propidium iodide (PI) double staining. HOS indicates untreated HOS cells; MOCK, HOS cells transfected with pCDNA-3.1; HOS+ING5, HOS cells transfected with pCDNA-3.1-ING5; MG63, untreated MG63 cells; MOCK, MG63 cells transfected with pCDNA-3.1; MG63+ING5, MG63 cells transfected with pCDNA-3.1-ING5.