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Western blot was used to detect the potential mechanisms of inhibitor of growth family of protein 5 (ING5) in osteosarcoma (OS) cells. Levels of p-Smad2, p-Smad3, t-Smad2 (total Smad2), t-Smad3 (total Smad3), and Smad4 were detected in ING5-expressing OS cells.
