Figure 3.

Pdgfrb-EGFP-, and Cspg4-DsRed expression in flat-mounted embryonic brain. Flat-mounted whole brains at E12.5 were immunostained to label vessels (CD31 or ColIV) and to amplify endogenous Cspg4-DsRed and Pdgfrb-EGFP fluourescence signals. Vessels were imaged en face at two depths: the pial surface (a) or the underlying periventricular vascular plexus (PVP, b–d), and in three locations: cortex (a and b), medial ganglionic eminence (MGE, c) or hindbrain (d). Note that localization of Cspg4-DsRed signal (red) and Pdgfrb-EGFP signal (green) in developing vascular mural cells is closely apposed to blood vessels labeled with CD31 (blue, a–c) or with ColIV (blue, d).