Figure 6.

Validation of genetically labeled developing vascular mural cells by immunofluorescence staining. Either Pdgfrb-EGFP (a–d)- or Cspg4-DsRed (e–h)-expressing mouse brain was prepared at E14.5 (a–c) and E16.5 (d–h), and immunofluorescence staining for PDGFRβ (a and e), NG2 (b and f), desmin (c and g) and CD13 (d and h) in combination with IB4 and DAPI was performed. Pdgfrb-EGFP⁺ cells lacking other marker staining (arrowheads, shown in c, d and h) and parenchymal Cspg4-DsRed⁺ cells (open arrowheads, e–g) are indicated. Note that CD13 expression was depicted only in the pial surface (asterisk, d). Postnatal cerebral cortex from the double Pdgfrb-EGFP; Cspg4-DsRed reporter mouse was stained for CD13 at P6 (i) and P14 (j).