Table 3.
Results of repeated-measures ANOVA of relative regional metabolite concentrations and the metabolite ratios Cho/tCr and Glx/tCr measured before and after the fasting, and from non-fasting controls, including Bonferroni-adjusted post hoc tests following significant (p < 0.05) overall test results.
| Braina | NAA |
tCr |
||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| region | F-test | p | p1 | P2 | Rateb | F-test | p | p1 | p2 | Rateb |
| RFL | F(2,28) = 6.4 | <0.05 | 0.001 | 1.00 | −4.1 | F(1.4,20) = 3.9 | < 0.05 | 0.025 | 1.00 | −3.7 |
| LFL | F(1.2,16) = 5.5 | < 0.05 | 0.000 | 0.75 | −5.1 | F(1.3,18) = 2.5 | 0.125 | |||
| RTL | F(2,28) = 2.8 | 0.080 | F(2,28) = 2.3 | 0.123 | ||||||
| LTL | F(2,28) = 4.8 | < 0.05 | 0.012 | 0.76 | −4.6 | F(2,28) = 2.2 | 0.125 | |||
| RPL | F(1.4,20) = 4.3 | < 0.05 | 0.002 | 0.18 | −4.5 | F(2,28) = 1.6 | 0.223 | |||
| LPL | F(2.28) = 1.6 | 0.222 | F(1.4,20)=1.1 | 0.322 | ||||||
| ROL | F(2,28)=0.7 | 0.487 | F(1.4,20)=0.9 | 0.379 | ||||||
| LOL | F(2,28)=2.1 | 0.134 | F(1.4,20)=1.5 | 0.250 | ||||||
| Cbl | F(2,28)=0.5 | 0.591 | F(2,28)=2.5 | 0.100 | ||||||
| Cho |
Glx | |||||||||
| RFL | F(1.5,20)=13.4 | < 0.05 | 0.000 | 0.91 | −11.3 | F(2,28)=26.3 | < 0.05 | 0.000 | 1.00 | −17.0 |
| LFL | F(2,28)=6.6 | < 0.05 | 0.001 | 1.00 | −11.9 | F(2,28)=20.9 | < 0.05 | 0.000 | 1.00 | −13.7 |
| RTL | F(2,28)=9.7 | < 0.05 | 0.004 | 0.87 | −9.0 | F(2,28)=26.0 | < 0.05 | 0.000 | 0.25 | −17.5 |
| LTL | F(1.3,18)=12.2 | < 0.05 | 0.000 | 1.00 | −11.9 | F(2,28)=13.6 | < 0.05 | 0.002 | 1.00 | −14.9 |
| RPL | F(1.4,20)=5.8 | < 0.05 | 0.000 | 1.00 | −10.3 | F(2,28)=29.2 | < 0.05 | 0.000 | 1.00 | −18.4 |
| LPL | F(1.2,17)=5.4 | < 0.05 | 0.000 | 1.00 | −9.5 | F(2,28)=27.2 | < 0.05 | 0.000 | 0.92 | −18.0 |
| ROL | F(2,28)=2.1 | 0.139 | F(2,28)=10.4 | < 0.05 | 0.003 | 1.00 | −18.3 | |||
| LOL | F(2,28)=6.0 | < 0.05 | 0.004 | 1.00 | −12.5 | F(2,28)=22.5 | < 0.05 | 0.000 | 1.00 | −20.8 |
| Cbl | F(2,28)=14.4 | < 0.05 | 0.001 | 1.00 | −10.6 | F(2,28)=18.0 | < 0.05 | 0.001 | 1.00 | −15.9 |
| Cho/tCr |
Glx/tCr | |||||||||
| RFL | F(2,28)=10.4 | < 0.05 | 0.002 | 1.00 | −7.62 | F(2,26)=12.1 | < 0.05 | 0.002 | 1.00 | −13.02 |
| LFL | F(1.8,26)=8.6 | < 0.05 | 0.010 | 1.00 | −7.25 | F(2,26)=8.6 | < 0.05 | 0.013 | 1.00 | −9.75 |
| RTL | F(1.3,18)=7.6 | < 0.05 | 0.058 | 0.28 | −4.67 | F(2,26)=16.8 | < 0.05 | 0.000 | 1.00 | −14.18 |
| LTL | F(1.4,19)=12.5 | < 0.05 | 0.000 | 1.00 | −10.36 | F(2,26)=4.6 | < 0.05 | 0.058 | 1.00 | −12.90 |
| RPL | F(2,28)=8.4 | < 0.05 | 0.006 | 1.00 | −7.33 | F(2,26)=20.1 | < 0.05 | 0.000 | 1.00 | −15.85 |
| LPL | F(2,28)=5.0 | < 0.05 | 0.007 | 1.00 | −6.58 | F(2,26)=13.2 | < 0.05 | 0.004 | 0.77 | −15.65 |
| ROL | F(2,28)=5.9 | < 0.05 | 0.008 | 1.00 | −7.50 | F(2,26)=8.5 | < 0.05 | 0.005 | 1.00 | −16.41 |
| LOL | F(2,28)=6.7 | < 0.05 | 0.007 | 1.00 | −9.39 | F(2,26)=19.6 | < 0.05 | 0.000 | 1.00 | −19.32 |
| Cbl | F(1.3,18)=10.6 | < 0.05 | 0.002 | 0.18 | −5.66 | F(2,26)=9.5 | < 0.05 | 0.004 | 1.00 | −12.39 |
Note: For mI no significant overall results were found. p is the overall significance estimated with ANOVA, p1 and p2 (significant if P1 or p2 < 0.05. In significant case the values of p1 or p2 were shown in bold) are significances of Bonferroni adjusted post hoc tests between pre- and post-fasting values (p1), pre- and non-fasting values (p2) that calculated for cases with p < 0.05.
Definition of the brain regions are the same as shown in Table 2.
Decreases of the metabolite values after fasting in ratio to pre-fasting values, calculated only when changes were significant in comparison to pre-fasting values (p1 < 0.05).