Table 1.

Experimental design and sample sizes for in vivo experiments.

Figure	Experiment	Day of experimentation (days post-stroke)	Sample size
1	Acute Western	3	4–5
1	TTC	3	12–16
2	Acute Western	0, 6 h, 12 h, 1, 3	5
2	Long-term Western	14	7–8
3	NeuN/BrdU for neurogenesis following suppression of Wnt activity	21	4–10
4	DCX/BrdU IHC for neuroblast proliferation	14	6–13
5	NeuN/BrdU IHC for peri-infarct neurogenesis	21	4–10
6	NeuN/GLUT1/BrdU IHC for hippocampal neurogenesis and angiogenesis	21	5
7	ColIV/BrdU IHC for angiogenesis	21	7
7	Laser Doppler for blood flow	0, 7, 14	5
8	Adhesive removal test	1, 4, 7, 14, 21, 28	12–16
8	Corner test	28	12–16

Note: Sample sizes for different groups were chosen based on preliminary studies that were performed with a small group of animals per group (n = 2 for Westerns and immunohistochemistry, and n = 3 for behavior). Using the difference in means (estimate of effect size) determined with the preliminary studies and the sample sizes used, priori power analysis was performed (two tailed, α error probability = 0.05, power (1−β error probability) = 0.8) using G × Power (version 3.1.9.2, Universitat Düsseldorf, Germany). Detailed sample sizes for each experimental group are specified in figure legends.