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Gating strategy used for the identification of cytokine producing T cells. a The gating strategy used to identify CD4 and CD8 T cells. b displays the memory subsets defined by CCR7 and CD45RA in CD4 and CD8 T cells. c, d Examples of cytokine upregulation (Pre and post vaccination, days 0 and 28 respectively) in CD8 and CD4 T effector memory (TEM) cells of a representative vaccinated volunteer
