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. 2018 Mar 14;15:13. doi: 10.1186/s12989-018-0249-1

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — PM exposure in infant mice enhances pulmonary inflammation in adulthood after induction of allergic asthma in vivo. a The exposure protocol to PM and induction of asthma model. PM (F1, F2 and F3: 50 μg per time; F1(s): 15 μg per time) or PBS was administered to juvenile BALB/c mice at 17, 19 and 21 days after birth by oropharyngeal aspiration (OA). An OVA-model of asthma was established at six weeks of age. b The test groups are shown in the table. c Total WBC and the differential counts for Monocyte, Neutrophil, Lymphocyte, Basophil and Eosinophil in BALF were evaluated by differential counting 24 h following the final protocol day. d Serum antibody concentration measured by ELISA. The data are expressed as the mean ± SEM of 5 mice per group. *p < 0.05 vs. Con group; #p < 0.05 vs. OVA group, ^&p < 0.05 vs. OVA/PM (F1, F2 or F3)