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. 2018 Mar 13;16:62. doi: 10.1186/s12967-018-1440-8

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© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Representative images showing quantification procedure using NewCAST™ Visiopharm^® software. Super image capture of a tissue slide (A); computer-assisted drawing of the whole tissue (green line) and manually drawn portal areas as regions of interest (ROI) in blue (B); selected areas within the ROIs by random sampling (grey squares) (C); counting frame within one field of view at 400 magnification (D)